Effect of Asparagine or Glutamine on Growth

ffects of L-asparagine and L-glutamine on growth, pigments content and metabolism of haseolus vulgaris plants, at two stages of plant growth and development in was investigated in icropropagated tissues (in vitro). In general, all growth parameters increased by 1 & 2 mM sparagine or glutamine treatments while decreased in response to other concentrations (3, 4 & 5 M), Similarly total carbohydrates and its fractions also increased in response to low oncentrations whereas the higher concentrations decreased these parameters, throughout the xperiment. The pattern of changes in chlorophyll a, chlorophyll b and carotenoids were more or ss similar to that in carbohydrates in response to asparagine or glutamine treatments. As ompared with untreated plants, treatment with all concentrations of asparagine or glutamine duced marked increases in amide nitrogen, total nitrogen and protein whereas ammonia itrogen, peptide nitrogen and total soluble nitrogen, decreased during seedling and vegetative tages. Ions content (K, Na, Ca and Mg) increased significantly by 1 mM asparagine or lutamine and markedly decreased by the other concentrations. Asparagine and glutamine eatments increased and decreased the growth promoters (auxins, gibberellins and cytokinins) vel at low and high concentrations, respectively. On the other hand a reverse situation was bserved in case of ABA content. In general, the activity of all the determined enzymes asparagine synthetase, glutamine synthetase, nitrate reductase and protease) was decreased by creasing asparagine or glutamine concentrations, during the two stages of Phaseolus vulgaris rowth and development.